ADN et chromosomes

Point mutation of bacterial artificial chromosomes by ET recombination.

Bacterial artificial chromosomes (BACs) offer many advantages for functional studies of large eukaryotic genes. To utilize the potential applications of BACs optimally, new approaches that allow rapid and precise engineering of these large molecules are required. Here, we describe a simple and flexible two-step approach based on ET recombination, which permits point mutations to be introduced i...

Rapid modification of bacterial artificial chromosomes by ET-recombination.

We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The procedure we describe here is rapid, was found to work with high efficiency and should be applicable t...

Conception et analyse des biopuces à ADN en environnements parallèles et distribués. (Design and analysis of DNA microarrays in parallel and distributed environments)

SMSDAdn - Motifs Séquentiels Discriminants pour les puces ADN

Discovering new information about groups of genes implied in a disease is still challenging. Microarrays are a powerful tool to analyze gene expression. They provide an expression level for genes under given biological situations. In this paper, we propose a novel approach outlining relationships between genes based on their ordered expressions. First, we propose to use a new material, called s...

Investigation into Pavement Curing Materials, Application Techniques, adn Assessment Methods

Different types of curing compounds were applied to lab and field concrete at different times after casting. Two application methods, single and double layer applications, were employed. Concrete properties, such as electrical conductivity, moisture content, sorptivity, degree of hydration, strength, and permeability were evaluated, and the results were employed for assessment of the curing eff...